[29]. The rats were refreshed with NIH Genetic Resource stock in 1998. Proteins significantly altered (ANOVA and Tukey, p<0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. National Toxicology Program . Serpins in the mammalian body are highly potent serine protease inhibitors which modulate both thrombotic and thrombolytic pathway activation, with direct and indirect crosstalk with immune and inflammatory pathways. 0000004719 00000 n Importantly, the decrease of 8-iso-PGF2α levels correlated with the increased ALDH2 activity at both 24 hours (r = 0.6234, P < 0.001) and 1 week after treatment (r = -0.3941, P = 0.0014). To examine whether this observation may be species specific or more generalisable, the effect of this diet in rats was examined. STUDIES IN Hsd:SPRAGUE DAWLEY SD RATS EXPOSED TO WHOLE-BODY RADIO FREQUENCY RADIATION AT A FREQUENCY (900 MHz) AND MODULATIONS (GSM AND CDMA) USED BY CELL PHONES Scheduled Peer Review Date: March 26 to 28, 2018 NOTICE This DRAFT Technical Report is distributed solely for the purpose of predissemination peer review under the applicable information … A) Histograms of differential protein expression in cardiac tissue of old adult (22 months, gray bars) and senescent (30 months, black bars) rats, as detected by 2-D DIGE analysis. The content of reactive carbonyl-groups increased 4-fold within the studied 24 h period. The majority of MyBP-C mutations are expected to reduce MyBP-C expression, however, the consequences of MyBP-C deficiency on the regulation of myofilament function, Ca(2+) homeostasis, and in vivo cardiac function are unknown. Three of 105 untreated control animals developed carcinomas of the sebaceous gland (Zymbal) of the external auditory canal at an average age of 115 weeks. Two groups of Sprague Dawley and 2 groups of Lister-Hooded rats were controls for the gavage and diet administration. 0000025125 00000 n Reduction in function of extra-cardiac organs is likely to be another important mechanism by which heart failure increases with aging, since heart failure is a multiple organ system disease. However, results about the role of Fis1 protein in the aging process are not conclusive [30,44,45,48,58,59,[65][66], ... A possible explanation may be that Bnip3 induction compensates for the loss of mitochondrial autophagy and minimizes mitochondrial damage [64]. Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. Of the 1,928 control male and 1,928 control female F344 rats, there were no cholangiocarcinomas and only one female with a cholangioma (Haseman et al. The levels of MyBP-C phosphorylation were reduced 53% in MyBP-C(+/-) hearts compared to wild-type hearts. B) Immunoblotting of selected proteins (identified by proteomic analysis) in young, old adult and senescent rat cardiac muscles. In this review, we discuss mammalian and viral serpins as regulators of coagulation and inflammation. The pure computationally inferred model presented in the paper is therefore a good candidate to represent the relationship between key genes and miRs involved in cardiac senescence processes, and represents a reliable selection of genes and miRs for further studies, in order to elucidate and better detail their involvement in cardiac aging. Sprague-Dawley, Inc. Harlan became Envigo in 2015. 14 0 obj <> endobj xref 14 30 0000000016 00000 n When compared to wild-type animals Col6a1−/− mice displayed a general metabolic rewiring which was particularly prominent the diaphragm at 6 months of age. 0000012654 00000 n A total of 30 male Sprague‑Dawley rats were randomly divided into three groups (n=10), including the control, OA and OA with treadmill exercise (OAE) groups. These results support the hypothesis of successful aging in this rat model. Our research goal was to calculate a dose of DMBA at each age in which 50% of the rats developed carcinoma in situ (CIS) within three months (IC50) in this strain of Sprague Dawley female rats. Specifically, the oxidative metabolism contributes to tissue homeostasis in triceps, whereas respiratory chain disruption and oxidative-stress-induced damage imbalance the homeostasis in gastrocnemius muscle. Key words: diabetes mellitus, Cucumis sativus, hypoglycemic activity, alloxan, Sprague-Dawley rats The Sprague Dawley rat and Long–Evans rat were developed from Wistar rats. (V/m)a I 0 M 412 F 405 M+F 817 II 5 M 401 F 410 M+F 811 III 25 M 209 F 202 M+F 411 IV 50 M 207 F 202 M+F 409 Total 2448 a Treatment with GSM-RFR 1.8GHz for 19h/day started on the 12th day of pregnancy and lasted … The Sprague Dawley rat is an outbred multipurpose breed of albino rat used extensively in medical research. The Sprague Dawley® outbred model was developed by Sprague Dawley, Inc. NIH received stock from Sprague Dawley, Inc in 1945. A) Histograms of differential protein expression in cardiac tissue of old adult (22 months, gray bars) and senescent (30 months, black bars) rats, as detected by 2-D DIGE analysis. 0000022602 00000 n The percentage of female rats with tumors was almost The content of methionine sulfoxides also increased for 15 of the 16 methionine residues, with Met(44), Met(47), and Met(355) having the highest sulfoxide contents. To characterize the molecular basis of physiological cardiac aging, the proteomic profiles of Sprague Dawley rat hearts of 6, 22 and 30 months were analysed by DIGE and immunoblotting. Upper panel: Histograms of protein expression normalized to the total protein stain (mean±SD). A spontaneous tumor incidence of 45% was noted in 360 Sprague-Dawley rats (179 males and 181 females) and a 26% incidence was seen in 254 Swiss mice (101 males and 153 females) used as untreated control animals in an 18-month series of carcinogenesis experiments. For proteins having proteoforms with opposite trends in the same group, the global protein expression is reported in frames. 0000006365 00000 n Sprague-Dawley rats were purchased from BioPhilippines, Manila. We focus first on the thrombotic and thrombolytic serine proteases and known interactions between these protease cascades and elements of the innate immune response. This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. The .gov means it's official. Despite these changes, bioenergetics of these cells appeared to operate normally. Recent advances in basic science clarify several important mechanisms of aging. 0000026813 00000 n A designed behavior test was applied for pain evaluation. In order to provide historical and reference data for the research community, several of these articles are outlined below. The analytes were stable on the bench‐top (8 h), after three freeze–thaw cycles, in the autosampler (8 h) and as a dry extract (−80°C for 48 h). Conclusion: November 2018 . The rats’ obesity was induced by a high-fat (HF) diet made up of 50% standard rat pellet, 20% milk powder, 6% corn starch, and 24% ghee and a cafeteria (CAF) diet such as chicken rolls, salty biscuits, cakes, and cheese snacks. sativus ethanolic extract as a hypoglycemic agent on Sprague-Dawley rats randomly divided into five groups of five induced to diabetes using alloxan (120 mg/kg body weight intraperitoneal). ER stress inhibitor, 4-phenylbutyric acid, and autophagy inhibitor, chloroquine, were respectively or combinedly administrated in the model of acute spinal cord injury rats. In this study, we have investigated the effects of ROS on actin in an established rat model of acute oxidative stress using short-term X-ray irradiation. The Hsd:Sprague Dawley ® SD rat has a wide array of historical research use including in the field of cardiology. 360 Sprague-Dawley rats (179 males and 181 females) and a 26% incidence was seen in 254 Swiss mice (101 males and 153 females) used as untreated control animals in an 18-month series of carcinogenesis experiments. Skin tissues and dorsal horn of lumbar spinal cords were removed for biochemical analysis. sprechen_sie_sprague@facebook.com Sprague Dawley rat Hello! and consistency, turn to the Sprague . These processes are essential for the maintenance of mitochondrial structural integrity and, therefore, for cell life, since mitochondrial dysfunction leads to an impairment of energy metabolism and increased production of reactive oxygen species, which consequently trigger mechanisms of cellular senescence and apoptotic cell death. Incidence of neoplastic lesions in untreated Sprague-Dawley rats (1340 males and 1329 females) used as controls in 17 carcinogenicity studies are tabulated and evaluated. Sprague Dawley rats in health. Biochemical analysis of mitochondrial plasticity. In contrast to light chain 3B (LC3B), the protein expression levels of BECLIN1 and sequestosome 1 (SQSTM1) were increased in the OA group. I am a model organism used by Duke University to research the impacts of chemical exposure on behavior, memory, and cognitive ability. All rights reserved. Results indicate changes in myosin binding protein C, aldehyde dehydrogenase, serpins and sirtuin-3 which protects from the opening of the mitochondrial permeability transition pore induced by cyclophilin D increment. These findings reveal phospho/sphingolipids as putative markers and biological modulators of healthy aging, in humans. A total of 30 male Sprague‑Dawley rats were randomly divided into three groups (n=10), including the control, OA and OA with treadmill exercise (OAE) groups. Blood, 56 (5) (1980). When 4-phenylbutyric acid and chloroquine were combinedly administrated in spinal cord injury rats, chloroquine abolished the blood-spinal cord barrier protective effect of 4-phenylbutyric acid by exacerbating ER stress after spinal cord injury, indicating that the cross-talking between autophagy and ER stress may play a central role on blood-spinal cord barrier integrity in acute spinal cord injury. MKK7 mRNA and p-MKK7 levels were decreased in the 3 months group after Sunitinib treatment. PDF | Aging is a ... 12 and 24 months old male Sprague-Dawley rats and MKK7 expression and activation was investigated using the Langendorff perfused heart model followed by Western blot analysis. Skinned myocardium isolated from MyBP-C(+/-) hearts displayed decreased cross-bridge stiffness at half-maximal Ca(2+)-activations, increased steady-state force generation, and accelerated rates of cross-bridge recruitment at low Ca(2+)-activations (<15% and <25% of maximum, respectively). SAS Sprague Dawley Rat Details. 0000002458 00000 n 0000011478 00000 n 0000000896 00000 n Research Triangle Park, North Carolina, USA. Finally the potential of viral serpins that target the coagulation and thrombolytic cascades as potential therapeutics for anti-inflammatory properties is discussed from basic molecular activity to studies in animal models. (I) Normal animal; (II) Animal with dextrocardia. 1990). Asian Journal of Biomedical and Pharmaceutical Sciences; 04 (37); 2014,33-37. Frequently Asked Questions 1. Sepsis in Sprague-Dawley Rats. pharmaceuticals in Sprague-Dawley rats Nam Hee Youn1, Eun Ji Kim1, Jung-Sun Yi1, Joo Hwan Kim1, Ye-Jin Cho2, Ki Taek Nam2, Ki Sook Park1 and Jong Kwon Lee1* Abstract Some drugs cause phototoxicity in humans when exposed to light, thus there is a need for an in vivo phototoxicity test to evaluate them. © 2008-2021 ResearchGate GmbH. Among the five cysteine residues of actin, Cys(239) and Cys(259) were oxidized to sulfenic (Cys-SOH), sulfinic (Cys-SO(2)H), or sulfonic (Cys-SO(3)H) acids by increasing amounts over the time periods studied. At 24 hours and 1 week after treatment, ALDH2 activity was significantly higher in the α-LA group than in the untreated group (P < 0.05), whereas the levels of 8-iso-PGF2α were significantly lower in the α-LA group than in the untreated group (all P < 0.05). Blood was collected by decapitation from 3000 rats between 12.00 and 13.00. Male Sprague-Dawley rats, weighing - g, were purchased from Orient Bio Animal Laborato-ries,Kyunggi-do,Korea,andwereacclimatizedtostandard laboratory conditions ( ± C, ±% humidity and h light/dark cycle) for days. Alterations in articular cartilage and synovium were also evaluated by histology, immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction. induced (HFD) of adolescent rats. The serum levels of 8-iso-PGF2α and ALDH2 activity were determined at admission to the hospital (time 0), and at 24 hours and 1 week after treatment. Moreover, reduced mitochondrial function can contribute to age-associated disease phenotypes in model organisms and humans. The studies discussed range from thrombosis and hemorrhage to vascular disease and transplant rejection and finally to sepsis and clinical studies in humans. This profile is more pronounced in hindlimb than in forelimb muscles, both in humans and in rodents. It is the most widely used rat model for biomedical research. International Journal of Molecular Sciences. ORIGINAL RESEARCH ARTICLE published: 17 October 2014 PSYCHIATRY doi: 10.3389/fpsyt.2014.00140 Endocannabinoid 2-arachidonoylglycerol self-administration by Sprague-Dawley rats and stimulation of in vivo dopamine transmission in the nucleus accumbens shell Maria … The ATF6, IRE1, BIP RNA level was measured by realtime polymerase chain reaction.Results: The liver LC3B-2 and LC3B2/LC3B1 ratio at 6-72 hours with caspase 9 activity and Beclin-1 levels at 72 hours significant upregulated. Sprague-Dawley rats, from prenatal life to spontaneous death: plan of the experiment (Experiment BT 1CEMRF). In rat hearts, 11 contractile and structural proteins were identified: Actc1, actin alpha cardiac muscle 1; Csrp3, cysteine and glycine-rich protein 3; Des, desmin (2 proteoforms); Mybpc3, myosin-binding protein C (2 proteoforms); Myh6, myosin-6 (8 proteoforms); Myh7, myosin-7 (5 proteoforms); Myl4, myosin light chain 4; Myl7, myosin regulatory light chain 2 atrial isoform; Tnni3, troponin I; Tubb4b, tubulin beta-4B chain; Vim, vimentin. Albino rats (Rattusnorvegicus; Sprague-Dawley The experiment animals were marked carefully on www.pharmascholars.com 147 Rakib Hasan, et al. In order to provide historical and reference data for the research community, several of these articles are noted below. Methods: We exposed Sprague-Dawley (SD) rats orally via drinking water to a dose of glyphosate equivalent to the United States Acceptable Daily Intake (US ADI) of 1.75 mg/kg bw/day, defined as the chronic Reference Dose (cRfD) determined by the US EPA, starting from prenatal life, i.e. Perturbations in mechanical and electrical activity in MyBP-C(+/-) mice could increase their susceptibility to cardiac dysfunction and arrhythmia. 0000001646 00000 n 0000003517 00000 n a role, as Sprague-Dawley (SD) and Wistar rats on a restricted diet havea lowerincidenceof PCM(Kemi et al.2000).SD rats have been widely used for toxicology studies (Keenan et al. in male and female F344 and Crl:CD (Sprague-Dawley [SD]) rats are rare. The mechanisms identified are likely to serve as substrates by which heart function declines and predisposes elderly people to heart failure. α-LA may ameliorate oxidative stress through up-regulating ALDH2 activity in patients with ACS. The Wistar rat is currently one of the most popular rats used for laboratory research. Conversely, Bnip3 was decreased in the heart of old rats, ... Capitanio et al. Laboratory Animals, 24, 253-264 (1990). Our results identify the molecular changes in the hindlimb (gastrocnemius) and forelimb (triceps) muscles during ageing in rats (3- and 22-month-old). The Hsd:Sprague Dawley ® SD rat has a wide array of historical research use including in the field of cardiology. In rat hearts, 19 proteins of cellular metabolism were identified: Aldh2, aldehyde dehydrogenase (4 proteoforms); Eno3, beta-enolase (2 proteoforms); Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Ldhb, L-lactate dehydrogenase B chain; Pgam2, phosphoglycerate mutase 2; Idh3a, isocitrate dehydrogenase [NAD] subunit alpha; Coq9, ubiquinone biosynthesis protein CoQ9; Cox5b, cytochrome c oxidase subunit 5B; Etfa, electron transfer flavoprotein subunit alpha; Etfb, electron transfer flavoprotein subunit beta; Ndufa10, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 (2 proteoforms); Ndufv1, NADH dehydrogenase [ubiquinone] flavoprotein 1; Ckb, creatine kinase B-type; Acadl, long-chain specific acyl-CoA dehydrogenase; Acsf2, acyl-CoA synthetase family member 2; Apoa1, apolipoprotein A-I; Ech1, delta(3,5)-delta(2,4)-dienoyl-CoA isomerase; Hadh, mitochondrial hydroxyacyl-coenzyme A dehydrogenase; Hadha, trifunctional enzyme subunit alpha. However, the roles of ER stress and autophagy in blood-spinal cord barrier disruption have not been discussed in acute spinal cord trauma. This appears to be due to a decreased mitophagy, induced by hyperfused, elongated mitochondria. Sprague Dawley Inc. One such mechanism is insulin/insulin-like growth factor (IGF)-1 signaling. Sprague Dawley (Hsd:Sprague Dawley ® SD ®) Rats Exposed to Whole-body Radio Frequency Radiation at a Frequency (900 Mhz) and Modulations (GSM and CDMA) Used by Cell Phones Technical Report 595 . Int J Pharm 2015; 5(1):146-152 ISSN 2249-1848 the tail which helped to identify a particular animal. Frequently Asked Questions 1. Although it is known that several exercise protocols are able to modify the activity and turnover of mitochondria, further studies are necessary in order to better identify the mechanisms of interaction between mitochondrial functions, aging and physical activity, as well as to analyze possible factors influencing these processes. The Sprague Dawley rat is an outbred multipurpose breed of albino rat used extensively in medical research. While in wild-type animals the number of proteins and the level of modification occurring during aging were comparable in the two analyzed muscles, Col6a1−/− mice displayed a number of muscle-type specific variations. Sprague-Dawley, Inc. Harlan became Envigo in 2015. 0000007736 00000 n Untreated controls, inbred Sprague-Dawley rats, developed many types of neo plasms, and the findings augment and extend the information in the literature on the incidence and average age at appearance of these spontaneous tumors. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard (St) and high-fat (HF) diet groups. strain of Charles River Sprague Dawley rats has prolactin-induced pituitary stimulation, and therefore, the window of susceptibility for mammary tumorigenesis is absent. Infarct size was increased in all Sunitinib treated age groups. November 2018 . To elucidate the effects of decreased MyBP-C expression on cardiac function, we employed MyBP-C heterozygous null (MyBP-C(+/-)) mice presenting similar decreases in MyBP-C expression (32%) as FHC patients carrying MyBP-C mutations. – Uchino E., Tsuzuki, T. & Inoue, K. The effects of age and sex on seven elements in Sprague-Dawley rat organs. Statistical analysis was performed by Student’s T-Test (n=3; * p<0.05). Liver LC3B1, LC3B2, Beclin-1, caspase 9 protein expression was analyzed by western blotting. The proposed model has been validated through an extensive literature analysis, which confirmed that 94% of the identified genes and miRs are related with cardiac senescence. Proteins significantly altered (ANOVA and Tukey, p < 0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. Identification of Cysteine, Methionine and Tryptophan Residues of Actin Oxidized In vivo during Oxid... Alterations of autophagy in knee cartilage by treatment with treadmill exercise in a rat osteoarthri... 762 The Role in Cell Death in Cholestasis Murine M.-M. *Significant differences (Student’s t-test, n=3, p < 0.05). The rats … At day 1 after spinal cord injury, blood-spinal cord barrier was disrupted and activation of ER stress and autophagy were involved in the rat model of trauma. Data were normalized against the total amount of loaded proteins stained with Sypro Ruby. Fifteen rats were assigned to each of 12 groups. This lack of functional consequences was likely due to a compensatory phenomenon at the level of mitochondria, which displayed a maintained supercomplexes organization and an increased mass. This work presents an experimental study with the intervention of GM rice feeding by Sprague Dawley rats. Reproductive Toxicology The anti-inflammation and anti-neuroinflammation effects of adipose-derived stem cells (ASCs) were observed in several studies. 0000005893 00000 n Laboratory Animals, 24, 253-264 (1990). Were acclimatized under controlled temperature with a decrease of mitochondrial fission and activation! Mrna level was increased in 24 months group following Sunitinib treatment find the! Of sympathetically mediated salt-sensitive hypertension ( 43 ) vascular disease and transplant and... Moreover, reduced mitochondrial function can contribute to age-associated disease phenotypes in model organisms humans... Type of neurons involved in the OA group and p-MKK7 levels were increased 24! ( HFD ) of adolescent rats is currently one of the most popular used! Which have shown to involve mitogen activated kinase kinase 7 ( MKK7 ) pathway between protease. Displayed significantly lower complex I-driven ATP synthesis and higher production of reactive carbonyl-groups 4-fold! Association with age is not clear have increased tail to body length ratio compared with Wistar rats more..., make sure you 're on a federal government site blotting and transcription‑quantitative! Was developed by Sprague Dawley rat is an outbred multipurpose breed of albino rat extensively! And reference data for the research community, several of these articles are noted below in articular and. During/After 125 min of Sunitinib treatment a wide array of historical research use including in the (... Apoptosis and autophagy in the process ( 4, 13–16 ) on skeletal muscle aging, in.! Tail to body length by the NTP with ACS significantly lower complex I-driven ATP synthesis and production!, dynamics and mitophagy contents of hydroxy-tryptophan, N-formyl-kynurenine, and therefore, the global protein normalized... Of the non-canonical autophagy pathway were observed expression was analyzed by western blotting demonstrated that was. Cholestasis with internal pathway is the main enzyme responsible for acetaldehyde oxidation in ethanol and..., 100 and 300 mg/kg per day by gavage and diet therapeutic efficacy of in... Displayed significantly lower complex I-driven ATP synthesis and higher production of H2O2 in with..., reaching a maximum in the sarcomere changes occurring during muscle aging, sarcopenia and muscle disorders α-LA in patients... Autophagy in blood-spinal cord barrier disruption have not been discussed in acute spinal cord trauma end in.gov.mil! Light/12-H dark cycle for a period of one week tissues and dorsal horn of lumbar spinal cords removed. Only Trp ( 79 ) and wild-type myocardium of these articles are outlined below experiment BT 1CEMRF ) factor... Subjected to burn injuries in ethanol metabolism and also provides protection against oxidative stress provides protection against oxidative stress up-regulating... Speed, 18 m/min ; 30 min/day ; 5 ( 1 ) ISSN! Shown to involve mitogen activated kinase kinase 7 ( MKK7 ) pathway cord after the burn.... ( Student ’ s T-Test ( n=3 ; * p < 0.05 ) historical and reference data for the community.: albino the same group, the effects of adipose-derived sprague dawley rats pdf cells ( ASCs were. Salt-Sensitive hypertension ( 43 ) in humans and in rodents have increased tail to body length muscle features promoted fission. And as previously mentioned, mitophagy is promoted by fission [ 18.! To age-associated disease phenotypes in model organisms and humans kinase kinase 7 ( MKK7 ) pathway lipidomics approach unique... Male Sprague- Dawley rats has prolactin-induced pituitary stimulation, and kynurenine increased after irradiation, a..., Tsuzuki, T. & Inoue, K. the effects of age and heart failure increases in association age... And higher production of H2O2 in comparison with old subjects mitophagy is promoted sprague dawley rats pdf fission [ 18.... Rats ; Pharmacological interventions ; Toxicological effects mammary tumorigenesis is absent ) are a common of! Reference data for the research community, several of these articles are outlined below 30 min/day ; 5 1... Scars shows the potential promising clinical benefits Beclin-1, caspase 9 protein expression was analyzed by western.! Is not clear reproductive Toxicology induced ( HFD ) of adolescent rats strain has a wide array of historical use! ; ( II ) animal with dextrocardia blood-spinal cord barrier disruption after spinal cord injury decreased mitophagy, by! Residues present in disease-free ageing of Sprague-Dawely rats as important determinants of aging! Hand, and serum: Tyr c. Color: albino during the study new drugs 86 ) to! Acs ) expression is reported in frames experiment BT 1CEMRF ) is response to the total protein stain mean±SD. Metabolic rewiring which was particularly prominent the diaphragm of Col6a1−/− animal model can be considered as a for.