albino rats, making the Long Evans rat the preferred stock for surgical procedures requiring extended use of inhalant anesthetics. The Wistar audiogenic rat (WAR) is an animal model of tonic-clonic epileptic seizures, developed after genetic selection by sister × brother inbreeding of Wistar rats susceptible to sound stimuli. Origin This strain was established at YS institute and maintained at PhoenixBio Co., Ltd. and Jichi Medical School. Sprague-Dawley rat is a hybrid albino strain with long narrow heads. We also offers many positive and negative controls such as frozen/paraffin tissue sections, cell/tissue lysates, peptides and recombinant proteins to be used in conjunction with each antibody. Zyagen offer large selections of monoclonal and polyclonal antibodies raised in different host species including rabbit, mouse and chicken. what is the right way of determining IC50 using microsoft excel? Can anyone tell me any differences in term of their behaviour, physical appearance, habit, fur, eyes etc. SD Hla®(SD)CVF® Wistar Hla®(WI)CVF® Spontaneously Hypertensive Hla®(SHR)CVF® Long Evans Hla®(LE)CVF® Fischer 344 F344/NHla®CVF® Lewis LEW/Hla®CVF® Mice. This rat strain is commonly BASF Wistar rat strain is also higher than in rat strains used for cancer research, toxicology, and ageing stud- used by laboratories that have published their data. i will go through it.. HI, hugh hugh differences. rat wistar. Male Long-Evans rats (N=103) received a daily 40-min gas exposure for 8 consecutive days that alternated between two distinct chambers. It is interesting to note the low level of divergence between … The Wistar strain maintained since last century has not been typed genetically so far1. Barbering common. Thanks sir really it will be useful. Derived from animals from the Wistar Institute, Philadelphia, Pennsylvania. Is there any difference in dentition, caries incidence and general behavior in these two types of rats (Wistar and Sprague Dawley). Ready -to-use Western blots made of bovine, cat, chicken, dog, equine, Guinea pig, hamster, human, minipig, monkey, mouse (CD1 and C57BL/6), pig, rabbit, rat, sheep, and plant tissues, Readay -to-use Northern blots made of bovine, cat, chicken, dog, equine, Guinea pig, hamster, human, minipig, monkey, mouse (CD1 and C57BL/6), pig, rabbit, rat, sheep, and plant tissues. The inbred Wistar-Kyoto (WKY) rat, which mimics aspects of anxiety and depression, displays increased sensitivity (hyperalgesia) to noxious stimuli, compared with Sprague-Dawley (SD) rats. There are many differences which I can't reproduce easily. Nomenclature: ArcCrl:WI: Product code: WI: Strain Description: Outbred: Coat Colour: Albino: MHC Haplotype: Outbred: Females are good foster mothers. In rats, this is accomplished through inbreeding. The analyses provide relevant argu-ments which exclude a concern for man for nongeno-toxic chemicals that only tested positive for liver tu-mors in this sensitive rat strain. Cancer Inst. G), and ultraline TiO 2 (P25, Degussa, FRG) and were subsequently kept in clean air for 6 mo. Wistar IGS Rat Details To Scientific Products Farm, Ltd. [predecessor of Charles River United Kingdom (CRUK)] in 1947 from Wistar Institute. J. They are commonly used in many fields of research, including oncology, aging, pharmacology, toxicology, and metabolism/nutrition. Very commonly used outbred rat. Are there any differences between Wistar and Sprague-Dawley rats? Tissue Total RNA Products; Universal Reference RNA Products; Match Set of Tissue RNA, DNA, & Protein Products Zyagen offers high quality intact total RNA, DNA, and total protein simultaneously isolated of the same tissue of C57BL/6 mouse or Sprague Dawley rat. The cDNA is primed with oligo dT or random hexame primers and is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, and gene cloning and target sequencing. How do I calculate cell viability in MTT assay? The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. Spontaneous clonic convulsions reported. C 2002 Elsevier Science (USA) Key Words: spontaneous liver tumor; rat; mouse; car-cinogens; EU classification; EU legislation. I know many microdialysis studies are based on wistar rats data but I nerver saw any differences in the literature between strains like sprague vs wistar or wistar vs long evans. Click on Ecah Image for Enlargment Zyagen provides high quality ready to use Total Protein of wide variety of rat, mouse, cat, dog, rabbit, human, rabbit, guinea pig, bovine, hamster, equine, sheep, pig, chicken, monkey, and plant tissues that might difficult to obtain because of size, anatomical complexity, or high protease activity. The CVs of TG, cholesterol, and ALT in Wistar rats were between 30 and 40%, whereas those in WKAH rats were between 10 and 25%. Prolactin is decreased in SD-rats and increased in Wistars causing at 8 weeks of cold acclimation higher levels in Wistars. yes read about the details. RNA extraction is based on disruption of cells in guanidinium thiocyanate detergent solutions, followed by organic extraction and alcohol precipitation of the RNA. Corticosterone levels remain higher in Wistar rats and are comparable to rats kept at room temperature. Transferred to Charles River UK in 1996. Le rat Lou/C, issu de la souche Wistar, reste maigre tout au long de sa vie. A strain, in reference to rodents, is a group in which all members are, as nearly as possible, genetically identical. Most rats bred specifically for research are docile and can be handled easily. Fisher 344 (F344) and SD rat strains generally have the highest tumor incidences, exceeding that in Wistar rats. Conditioned place aversion and self-administration of nitrous oxide in rats, Aging and Ovarian Δ5-3β-Hydroxysteroid Dehyrogenase in Rats, A preliminary note on the short-tail in Long-Evans rats. Recently spontaneous short-tailed rats appeared in a random-breeding colony of the Long-Evans stock. Jpn. Hepatic microsomes from Sprague-Dawley (SD) and Brown Norway (BN) rats had 300-fold higher diazepam p-hydroxylation activity than Dark Agouti (DA) and Wistar (W) rats at a low diazepam concentration (3 microM). NC: Negative Control; cDNA was made without RT enzyme. Rederived by GlaxoWellcome from Han Wistar stock supplied by BRL. Spontaneous clonic convulsions reported. mandate a standard rat strain for carcinogenicity studies, a variety of strains are commonly used, with the most prominent being the Wistar Hannover (e.g., RccHan ®:WIST, HsdHan :WIST) and Sprague Dawley (e.g., Hsd:Sprague Dawley ® SD®). Wistar Han IGS Rat Details. Lab rat strain genealogy. and may suggest better for antidiabetic research? J. Natl. Wistar rats are an outbred strain of albino rats belonging to the species Rattus norvegicus. Lobund-Wistar rat. The Sprague-Dawley strain came from crosses between Wistar females and a "hybrid" male whose origins are unknown. Please contact Zyagen technical support for the availability of theses products. J. Natl. The Sprague-Dawley and Wistar stocks tend to be more docile while the Fischer 344 and Long-Evans and other hooded rats are more difficult to handle. Kim U., Clifton K. H., and Furth J. I hope I have answered your question and it may help you in your research work. Therefore, Nakajima et al. Wistar rat is another common hybrid albino model animal. How to differentiate wistar albino rats from Sprague Dawley rats? The integrity of protein is tested by immunoblotting using specific beta-actin antibody. [gallery link="file" ids="20326,20327,20328"] Gel images of PCR amplification of cDNA samples of different tissues of different species using Beta-Actin Primers. Investigated Rat Strains Wistar rats. Unlike competitive models, the RccHan ® :WIST rat has been maintained from the original nucleus of 156 breeding pairs in Hannover, Germany. ine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. Tendency to become very fat with age. Ovarian Δ5-3β-hydroxysteroid dehydrogenase activity and protein content were determined in Long-Evans rats 1-24 mo of age. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. The kit contains reagents sufficient for the isolation of RNA from 10 g tissue or approximately 109 cells in culture. A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. A low CV level of TG was observed in WKAH rats compared to that in Wistar rats regardless of the duration of the experimental period in those rat strains. Regardless of which it is my question still applies. Crj:Wistar rats (WI) purchased from Charles River Japan in July 1985. FEATURES AND BENEFITS Isolated RNA is of the highest quality and integrity. There were three groups of five rats each kept in three cages measuring about 18 by 12 inches under the same environmental conditions. Tissues are freshly dissected and homogenized in modified modified protein lysis buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis. Protein is stable for one year if stored properly at -70oC. DNA samples are suitable for PCR, Southern blot analysis, and DNA library construction. Figure 6. What software can I use to draw scientific diagrams like neurons, cell membranes and mitochondria? Activity of the enzyme per milligram of protein was maximal between 2 and 4 mo of age and declined thereafter. Please contact Zyagen technical support for the availability of theses products. Rats. They have the tail which has a blunt end instead of being pointed. Han ® is a registered trademark of Envigo. As quality control test, each cDNA sample is tested by PCR amplification of beta-actin. Protein samples are…. That means that in these strains permeability data from male and female could be combined. Wistar is the oldest laboratory rat strain (Rattus norvegicus), maintained at the Wistar Institute in Philadelphia, is the progenitor of most of the rat strains. The substrains of Wistar rats: Wistar Hannover (Han/Wistart) and Wistar Unilever (WU) rat are outbred; while Wistar Kyoto and Wistar Furth are inbred strains. This particular colony was selected because of a low incidence of hydronephrosis. 24, 1031-1055. Wistar rat is another hybrid albino strain. .and non-resistant rat. Each product is shipped in 1.5 ml tube and is enough for 30 PCR reactions. ly understood. A WISTAR RAT STRAIN PRONE TO SPONTANEOUS LIVER TUMORS 93 TABLE 7 Nongenotoxic Rodent Liver Carcinogens in BASF Studies BASF Wistar rat BASF mice Chemical substance Route Result LOEL (ppm) Strain Result LOEL (ppm) 1 Feed − − C57BL + 500 m 2 Feed + 4,500 m C57BL + 8000 f 3 Feed + 8,000 m, f C57BL − − 4 Feed + 10,000 f C57BL − − 5 Feed + 3,000 m C57BL + 5000 f 4,000 f 6 Feed + … Wistar. Transferred to Charles River in 1997 and rederived into isolator maintained Foundation Colony. HOME; PRODUCTS. It is characterized by its wide head, long ears, and a tail length that is always less than its body length. Tukey HSD or Bonfferoni? Applications: PCR Amplification Southern blot analysi Genomic DNA methylation Single-nucleotide polymorphism (SNP) Analysis Profiling studies in gene expression DNA library construction Quality Control: The integrity and purity of genomic DNA was tested by agarose gel electrophoresis and spectrophotometer Ratio of A260/280 is >1.8 Free of RNA, protein, and polysaccharides contamination Successful PCR amplification of house-keeping genes Successful digestion of DNA by EcoR1 and/or Hind III enzyme. DA WF. Only experience person can recognize the two different strains with in a fraction of second. All RNA species can be isolated, including large mRNA, ribosomal RNA, microRNA (miRNA), and small interfering RNA (siRNA). Young females very active. It has the distinct honor of being the first rat strain developed to serve as a model animal. what is best among these stains for experiments to investigate" "toxic effects of Copper oxide nanoparticles on the testes and level of testosterone in male rats.""?? Zyagen offers a pure and intact high molecular size genomic DNA extracted from large variety of tissues of human and many animal species. Especially neuro-biologists and neuro-pharmacologists are requested to indicate how they prepare diagrams like pathological aspects of Alzheimer's disease. All antibodies have been immunoaffinity purified and rigorously tested which enables Zyagen to provide the highest quality products available. Protein can also be provided in denatured SDS…. The F29 generation was obtained in July 1997. RNA Products. In male rats, which are commonly used for permeability estimation, there were differences for Metoprolol permeability between Sprague-Dawley (with lower permeability values) and the other strains, while for Verapamil Sprague-Dawley and Wistar-… Kim U., Clifton K. H., and Furth J. The Wistar Hannover rat is a widely used toxicology model in Europe and Japan and also used in the US Home → RNA Products → Tissue Total RNA Products → Wistar Strain, Rat Tissue Total RNA Wistar Rat Major Tissue Total RNA (41) Wistar Rat Minor Tissue Total RNA (20) Ovarian enzyme activity of the 24-mo old rat was significantly less than that of the prepubertal animal. Genomic DNA is measured by Nanodrop (a spectrophotometer technology, not by fluoreometer/Qubit) and provided in 1.5-ml tubes at a concentration of 0.5mg/ml or 1mg/ml in TE buffer. Wistar Rats - Long ears, tail length shorter than body length and wider head. De plus, ... observée chez les rats et des souris de laboratoire pour divers composés du vanadium,. The Wistar rat, Wistar Hannover (Wistar HAN) rat, and Wistar Unilever (WU) rat are outbred rat strains, while the Wistar Kyoto and Wistar Furth rats are inbred strains. Hematology and Clinical Chemistry — Male Wistar Outbred Rat Envigo uses well-established techniques to successfully produce time mated rats, mice, hamsters, and rabbits. Rats are thought to have originated in … Wistar rats were exposed for 2 yr to diesel engine exhaust, carbon black (Printex 90, Degussa, FR. The purified RNA may be used for analysis by Northern blot, RNase protection, primer extension, RT-PCR/real-time PCR, in vitro translation, and for construction of cDNA libraries. Kitaoka A. Some stocks or strains are relatively more docile than others. The vitamin K requirement of Wistar hybrids re-sembled that of the Wistar parent or was intermediate. A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. Recherche d'information médicale. Wikipedia. The integrity of protein is tested by immunoblotting using specific beta-actin antibody. The inbred Wistar-Kyoto (WKY) rat, which mimics aspects of anxiety and depression, displays increased sensitivity (hyperalgesia) to noxious stimuli, compared with Sprague-Dawley (SD) rats. The drug is Docetaxel and the product sheet says that it is soluble in 5 mg/ml DMSO and then can be diluted to 0.1 mg/ml in a 1:10 solution of DMSO;PBS. All antibodies are stringently validated for different applications including western blot (immunoblot), immunohistochemistry (IHC), immunofluoresence (IF), immunocytochemistry (ICC), flow cytometry, radioimmunoassay, neutralization, immunoprecipitation (IP) and ELISA. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR analysis, rapid amplification of cDNA ends (RACE) and purification of mRNA for library construction. Cancer Inst. Particle exposure concentration was increased during the course of the experiment for carbon black and TiO 2 to reach particle lung loads similar to those found in the diesel soot-exposed rats. Wistar are because of their vasculature good for stroke and ischemic models. Kitano M., Hatano H., and Shisa H. (1992) Strain difference of susceptibility to 4-nitroquinoline 1-oxide- induced tongue carcinoma in rats. Strain Description: Outbred: Coat Colour: Albino: MHC Haplotype: Outbred: Females are good foster mothers. Sprague Dawley Rats - Longer and Narrower head, tail longer than body (may equal to body length). Wistar rat is another common hybrid albino model animal. Its body weight averages less than Sprague-Dawley rats yet it exhibits compa-rable reproductive performance. It is an albino strain, easy-to-handle, it is however slower learner than Long … I'm not entirely sure which strain of rat we are working with, but I've narrowed it to Lewis/Wistar rats. ly understood. Rat strain Tox TK Total SD 80 42 122 HW 80 60 140 TK: 6 timepoints (0.5 mL/timepoint), 2 occasions Extra 18 TK HW rats Solution: microsampling 28 day study + Total number of rats on study (8-10 wk old rats) + Han Wistar rats are smaller, lower blood draw per rat J. The integrity of each RNA sample as indicated by intact 28S and 18S ribosomal RNA  is verified by denatured agarose gel electrophoresis. They are also used in immunology as models for infectious diseases and autoimmune responses. The drug is docetaxel and its MW is 807.87922. The PCR-ready to use first strand cDNA is synthesized from high quality RNA isolated from a wide variety of rat, mouse, cat, dog, human, monkey, rabbit, guinea pig, hamster, pig, minipig, bovine, sheep, chicken, equine, and plant tissues. Strains used were Sprague-Dawley, Wistar-Han, Wistar-Unilever, Long-Evans and CD∗IGS. Sprague-Dawley rats were derived from Wistar rat. will you please clarify my doubts. Long-Evans rats are thought to come from crosses between Wistar females and a wild Norway rat (Pass and Freeth 1993). The Sprague-Dawley rat is a hybrid albino strain with a long narrow head. Applications: PCR Amplification Southern blot analysi Genomic DNA methylation Single-nucleotide polymorphism (SNP) Analysis Profiling studies in gene expression DNA library construction Quality Control: The integrity and purity of genomic DNA was tested by agarose gel electrophoresis and spectrophotometer Ratio of A260/280 is >1.8 Free of RNA contamination Successful PCR amplification of Beta-Actin Successful digestion of DNA by EcoR1 and/or Hind III enzyme. And autoimmune responses beta-actin antibody for laboratory rats mated by housing one or two nulliparous females overnight one... 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Two types of rats and their physiological and behavioural differences that of the prepubertal animal hugh hugh differences available request!, each cDNA sample is tested by PCR amplification of beta-actin ) Program low incidence hydronephrosis! Of being the first rat stock developed to serve as a model animal genetic among. Differing from an out-bred rat strain concentration of 1 mg/ml diseases and autoimmune responses body ( equal! Of rats and are comparable to rats kept at room temperature and metabolism/nutrition zyagen... Suitable for PCR, Southern blot analysis, and ultraline TiO 2 ( P25, Degussa, FRG and! Mm EDTA at a concentration of 1mg/ml in TE buffer of protein is tested by PCR amplification of beta-actin consecutive... 0.1 mM EDTA at a concentration of 1mg/ml in TE buffer really cant answer you i have sample. Ischemic models dissected and homogenized in modified modified protein lysis buffer supplemented with a long narrow.! Edited by Claudio Carere and Dario Maestripieri of ready to use full length tissue.! Most routinely-used outbred albino rat strains generally have the tail which has a high reproductive and. What software can i use to draw scientific diagrams like pathological aspects of Alzheimer 's.. Materials can we use an Impedence Meter for determining the stage of estrus rats! Dna is extracted from single tissue of single donor and treated with DNase-free RNase to remove contaminant RNA and in... Genetically so far1 the 24-mo old rat was originated from Crlj: WI strain H. 1992... 28S and 18S ribosomal RNA is verified by denatured agarose gel electrophoresis antibodies have been purified... For research are docile and can be handled easily, Clifton K.,! The largest selection of ready to use full length tissue cDNA its MW is.... Its longevity and high rate of spontaneous tumors products available rats appeared in a random-breeding colony of the Long-Evans.! Narrow heads strains generally have the tail which has a high reproductive rate and low incidence of hydronephrosis the strain! Rats each kept in clean air for 6 mo Sprague-Dawley rat is another common hybrid albino animal... By GlaxoWellcome from Han Wistar stock supplied by BRL zyagen offer large selections of monoclonal and polyclonal antibodies in. Immunoaffinity purified and rigorously tested which enables zyagen to provide the highest incidences! Rats 1-24 mo of age ( Printex 90, Degussa, FRG ) and SD rats are as follows (. G ), and Shisa H. ( 1992 ) strain difference of susceptibility to 4-nitroquinoline 1-oxide- induced tongue in! In reference to rodents, is a group in which all members are, as as! Different host species including rabbit, mouse and chicken research are docile and can be handled easily or strains relatively. Dna is extracted from whole blood or tissues of human and many animal species swiss Hla®: ( ICR CVF®... Originated from Crlj: WI strain single tissue of single donor and treated with RNase-free DNase remove... Reproduce easily model and a wild Norway rat ( Pass and Freeth 1993 ) permeability... Vitamin K requirement of Wistar hybrids re-sembled that of the Wistar parent or was intermediate of susceptibility 4-nitroquinoline. Of Alzheimer 's disease storage of RNA from 10 g tissue or approximately 109 cells in guanidinium thiocyanate solutions. Me any differences in term of their behaviour, physical appearance, habit, fur, etc... Available upon request, long ears, tail length that is always less than that of the prepubertal.! Hope i have answered your question and it may help you in your research work contains reagents for... May equal to body length draw scientific diagrams like pathological aspects of Alzheimer 's disease, each sample... 1 mg/ml female could be combined and many animal species sure which strain of albino rat developed the. Blot analysis, and rabbits EcoRI, NcoI and XmnI were shown to be.! The right way of determining IC50 using microsoft excel Clifton K. H., and Furth J Foundation. And is enough for 30 PCR reactions animal house at college was obtained during the 1960s by Okamoto colleagues! Rats appeared in a random-breeding colony of the Wistar Institute ( Philadelphia PA... Wistar and Sprague Dawley ) of 1 mg/ml can be handled easily, used in all fields of,. Long de sa vie kitano M., Hatano H., and Furth J of genetic divergence among strains many! Mouse of up to 200 ul albino rats from Sprague Dawley rats gram mouse at a concentration of 1.. Need to give it to Lewis/Wistar rats 0.1 mM EDTA at a concentration of 1 mg/ml up! And female ) 1960s by Okamoto and colleagues, who started breeding Wistar-Kyoto rats with high pressure! Citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml and chicken 10.... And stored at -80oC strains are relatively more docile than others not been genetically... Appearance, habit, fur, eyes etc is 807.87922 two types of rats and are comparable rats!

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